Why is this Sub so dark?

Hi,
this is not an SGP issue, I think, but since we do have a large number of experienced users here, I thought I would just ask for some help.

I’m using an ASI183MMP with a TSAPO81Q to photograph NGC6823 in narrow band. The last clear night was a few weeks ago and the HA Subs were good - see link below.

Last night I had another clear night, but the results were totally different: As can be seen in the second sub, the stars can be seen, but the HA data is completely black. Same setup, same Gain and Offset and since the stars are there, the cap was not on.

Does anybody know what the cause could be? My intuition says, probably due on the lens, as the subs got darker as the night progresses.

Thank you for your feedback in advance.

Regards,
Allen

Starts at line 1727 - 23:17, to be prcise. Thanks.